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Luis G. Brieba (CV)

Professor (3-D, SNI 2)

Luis G. Brieba (CV)


Professor (3-D, SNI 2)


Structural Biochemistry


lgbrieba (at) langebio cinvestav mx


Office: 52 (462) 166-3007
Lab: 52 (462) 166-3103


2002-2005: Postdoctoral fellow, Harvard Medical School


1997-2001: Ph.D., Biochemistry Department, U. of Texas Health Science Center at San Antonio
1989-1993: B.A., ITESM Campus Guaymas


2005: Howard Hughes International Scholar
2003: Pew Latinoamerican Fellow
2003: Guarino Award for academic excelence
1997: Fulbright-Garcia Robles Fellow

1: Cardona-Felix CS, Lara-Gonzalez S, Brieba LG. Structure and biochemical
characterization of proliferating cellular nuclear antigen from a parasitic
protozoon. Acta Crystallogr D Biol Crystallogr. 2011 Jun;67(Pt 6):497-505. Epub
2011 May 12. PubMed PMID: 21636889.

2: Arvizu-Gómez JL, Hernández-Morales A, Pastor-Palacios G, Brieba LG,
Alvarez-Morales A. Integration Host Factor (IHF) binds to the promoter region of
the phtD operon involved in phaseolotoxin synthesis in P. syringae pv.
phaseolicola NPS3121. BMC Microbiol. 2011 May 4;11:90. PubMed PMID: 21542933;
PubMed Central PMCID: PMC3112066.

3: de-la-Re-Vega E, Garcia-Orozco KD, Arvizu-Flores AA, Yepiz-Plascencia G,
Muhlia-Almazan A, Hernández J, Brieba LG, Sotelo-Mundo RR. White spot syndrome
virus Orf514 encodes a bona fide DNA polymerase. Molecules. 2011 Jan
12;16(1):532-42. PubMed PMID: 21228759.

4: Casados-Vázquez LE, Lara-González S, Brieba LG. Crystal structure of the
cysteine protease inhibitor 2 from Entamoeba histolytica: functional convergence
of a common protein fold. Gene. 2011 Jan 15;471(1-2):45-52. Epub 2010 Oct 15.
PubMed PMID: 20951777.

5: Pastor-Palacios G, Azuara-Liceaga E, Brieba LG. A nuclear family A DNA
polymerase from Entamoeba histolytica bypasses thymine glycol. PLoS Negl Trop
Dis. 2010 Aug 10;4(8):e786. PubMed PMID: 20706627; PubMed Central PMCID:

6: Cardona-Felix CS, Pastor-Palacios G, Cardenas H, Azuara-Liceaga E, Brieba LG.
Biochemical characterization of the DNA ligase I from Entamoeba histolytica. Mol
Biochem Parasitol. 2010 Nov;174(1):26-35. Epub 2010 Jul 24. PubMed PMID:

7: Meneses E, Cárdenas H, Zárate S, Brieba LG, Orozco E, López-Camarillo C,
Azuara-Liceaga E. The R2R3 Myb protein family in Entamoeba histolytica. Gene.
2010 May 1;455(1-2):32-42. Epub 2010 Feb 12. PubMed PMID: 20156532.

8: Brieba LG. Template dependent human DNA polymerases. Curr Top Med Chem.
2008;8(15):1312-26. Review. PubMed PMID: 18991720.

9: Brieba LG. Nucleic acid metabolism and human disease. Curr Top Med Chem.
2008;8(15):1311. PubMed PMID: 18991719.

10: Quintas-Granados LI, Orozco E, Brieba LG, Arroyo R, Ortega-López J.
Purification, refolding and autoactivation of the recombinant cysteine proteinase
EhCP112 from Entamoeba histolytica. Protein Expr Purif. 2009 Jan;63(1):26-32.
Epub 2008 Sep 14. PubMed PMID: 18824105.

11: Mejia-Castillo T, Hidalgo-Lara ME, Brieba LG, Ortega-Lopez J. Purification,
characterization and modular organization of a cellulose-binding protein, CBP105,
a processive beta-1,4-endoglucanase from Cellulomonas flavigena. Biotechnol Lett.
2008 Apr;30(4):681-7. Epub 2007 Nov 6. PubMed PMID: 17985078.

12: Kim TW, Brieba LG, Ellenberger T, Kool ET. Functional evidence for a small
and rigid active site in a high fidelity DNA polymerase: probing T7 DNA
polymerase with variably sized base pairs. J Biol Chem. 2006 Jan
27;281(4):2289-95. Epub 2005 Nov 27. PubMed PMID: 16311403.

13: Brieba LG, Kokoska RJ, Bebenek K, Kunkel TA, Ellenberger T. A lysine residue
in the fingers subdomain of T7 DNA polymerase modulates the miscoding potential
of 8-oxo-7,8-dihydroguanosine. Structure. 2005 Nov;13(11):1653-9. PubMed PMID:

14: Guo Q, Nayak D, Brieba LG, Sousa R. Major conformational changes during
T7RNAP transcription initiation coincide with, and are required for, promoter
release. J Mol Biol. 2005 Oct 21;353(2):256-70. PubMed PMID: 16169559.

15: Brieba LG, Eichman BF, Kokoska RJ, Doublié S, Kunkel TA, Ellenberger T.
Structural basis for the dual coding potential of 8-oxoguanosine by a
high-fidelity DNA polymerase. EMBO J. 2004 Sep 1;23(17):3452-61. Epub 2004 Aug 5.
PubMed PMID: 15297882; PubMed Central PMCID: PMC516626.

16: Mukherjee S, Brieba LG, Sousa R. Discontinuous movement and conformational
change during pausing and termination by T7 RNA polymerase. EMBO J. 2003 Dec
15;22(24):6483-93. PubMed PMID: 14657021; PubMed Central PMCID: PMC291813.

17: Brieba LG, Ellenberger T. Hold tight (but not too tight) to get it right:
accurate bypass of an 8-oxoguanine lesion by DNA polymerase beta. Structure. 2003
Jan;11(1):1-2. PubMed PMID: 12517331.

18: Mukherjee S, Brieba LG, Sousa R. Structural transitions mediating
transcription initiation by T7 RNA polymerase. Cell. 2002 Jul 12;110(1):81-91.
PubMed PMID: 12150999.

19: Brieba LG, Padilla R, Sousa R. Role of T7 RNA polymerase His784 in start site
selection and initial transcription. Biochemistry. 2002 Apr 23;41(16):5144-9.
PubMed PMID: 11955062.

20: Brieba LG, Sousa R. T7 promoter release mediated by DNA scrunching. EMBO J.
2001 Dec 3;20(23):6826-35. PubMed PMID: 11726518; PubMed Central PMCID:

21: Brieba LG, Sousa R. The T7 RNA polymerase intercalating hairpin is important
for promoter opening during initiation but not for RNA displacement or
transcription bubble stability during elongation. Biochemistry. 2001 Apr
3;40(13):3882-90. PubMed PMID: 11300767.

22: Brieba LG, Gopal V, Sousa R. Scanning mutagenesis reveals roles for helix n
of the bacteriophage T7 RNA polymerase thumb subdomain in transcription complex
stability, pausing, and termination. J Biol Chem. 2001 Mar 30;276(13):10306-13.
Epub 2000 Dec 21. PubMed PMID: 11124963.

23: Huang J, Brieba LG, Sousa R. Misincorporation by wild-type and mutant T7 RNA
polymerases: identification of interactions that reduce misincorporation rates by
stabilizing the catalytically incompetent open conformation. Biochemistry. 2000
Sep 26;39(38):11571-80. PubMed PMID: 10995224.

24: Brieba LG, Sousa R. Roles of histidine 784 and tyrosine 639 in ribose
discrimination by T7 RNA polymerase. Biochemistry. 2000 Feb 8;39(5):919-23.
PubMed PMID: 10653635.

25: Gopal V, Brieba LG, Guajardo R, McAllister WT, Sousa R. Characterization of
structural features important for T7 RNAP elongation complex stability reveals
competing complex conformations and a role for the non-template strand in RNA
displacement. J Mol Biol. 1999 Jul 9;290(2):411-31. PubMed PMID: 10390341.

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