METABOLOMICS AND MASS SPECTROMETRY

doctor

More about us:

Dr. José Juan Ordaz Ortiz

Principal Investigator

Metabolomics is the study of small molecules or metabolites (metabolome) in a cell, tissue or whole organism. These types of methods are complementary to genomic, transcriptomic and proteomic analysis for the molecular characterization of biological systems and changes within these systems. Metabolomics is also defined as the comprehensive analysis of all compounds with low molecular weight (< 1500 Da) in a biological system or its parts; for example, the extract of a plant root. The plant metabolome comprises of a wide range of chemical species with diverse physical properties; from inorganic ionic compounds to hydrophilic carbohydrates, organic acids and amino acids and a range of related hydrophobic compounds like lipids. The plant metabolome has received more attention in recent years due to its importance in health, food and nutrition, the study of food safety and crop improvement.

Research lines:

  • Development and application of a range of chromatography and mass spectrometry methods, for the structural analysis of biologically active molecules in order to understand their mechanism of action at the molecular level.
  • Discovery of new metabolites and quantitative metabolomics.
  • Metabolite profiles such as: non-structural carbohydrates, semi-volatile, volatile and non-volatile organic compounds, plant hormones and phenylpropanoids.
  • Characterization of bioactive compounds from plant extracts.
  • Plant cell wall polysaccharides, structural analysis using MS, GC-MS and enzymatic hydrolysis, as well as immunodetection using specific molecular probes.

Recent projects:

  • Metabolome and transcriptome comparison in Capsicum.
  • Ferulic acid oligomer profiles and its natural variation in corn kernels.
  • Metabolome and transcriptome comparison of vanilla from different regions of Mexico.
  • Development of MALDI-MS Imaging for the localization of cytokinins in Arabidopsis thaliana.
  • Non-targeted metabolomic analysis of a soil.

Most relevant publications:

  • Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites. Planta, 2017, 247 (2): 405-412.
  • Genomic history of the origin and domestication of common bean unveils its closest sister species. Genome Biology, 2017,18(1):60.
  • Cyclopurpuracin, a cyclopeptide from Annona purpurea seeds. Phytochemistry Letters, 2018, 23:164–167.
  • Homoisoflavonoids Are Potent Glucose Transporter 2 (GLUT2) Inhibitors: A Potential Mechanism for the Glucose-Lowering Properties of Polygonatum odoratum. Journal of Agricultural and Food Chemistry, 2018, 66(12): 3137-3145.
  • Isolation, identification, and ecology of growth and taxol production by an endophytic strain of Paraconiothyrium variabile from English yew trees (Taxus baccata). Fungal Biology, 2015, 119 (11): 1022-1031.
  • Developing Pericarp of Maize: A Model to Study Arabinoxylan Synthesis and Feruloylation. Frontiers in Plant Science, 2016, 7:1476:1-20.
  • Cell wall microstructure analysis implicates hemicellulose polysaccharides in cell adhesion in tomato fruit pericarp parenchyma. Molecular Plant, 2009, 2(5): 910-921.
  • Classification of wheat varieties based on structural features of arabinoxylans as revealed by endoxylanase treatment of flour and grain. Journal of Agricultural and Food Chemistry, 2005, 53: 8349-8356.
  • Assessing temporal flux of plant hormones in stored processing potatoes using high definition accurate mass spectrometry. Horticulture Research, 2015. 2: 1-8.
  • Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana. Journal of Experimental Botany, 2015, 66 (13): 3893–3905.

Recent publications:

To access all publications, click the following links:

Dr. José Juan Ordaz Ortiz

Principal Investigator

jose.ordaz.ortiz@cinvestav.mx

M. Sc. Ana Laura Guzmán Ortiz

Research Technician

“Metabolomic services assistant”

ana.guzman@cinvestav.mx

Dr. David Gómez Zepeda

Postdoc

“Proteome comparison in Capsicum fruit”

david.gomez.zepeda@cinvestav.mx

M. Sc. Felipe de Jesús Cervantes Hernández

PhD student in Plant Biotechnology

"Metabolomic and transcriptomic data integration in dynamic network models during the development of the chili pepper fruit (in codirection with Dr. Octavio Martínez)”

felipe.cervantes@cinvestav.mx

Josué Esaú Macias López

Master’s student in Integrative Biology

"The influence of the environment in the metabolomic profiles in vanilla (Vanilla planifolia) in different stages of fruit development"

josue.macias@cinvestav.mx

Héctor Rogelio Nájera Gómez

Master’s student in Plant Biotechnology

"Development of MALDI Imaging and immunohistochemical methods to localize phytohormones during the development of the gynoecium (in codirection with Dr. Stefan de Folter)”

hector.najera@cinvestav.mx

Oscar Fernández Fernández

Master’s student in Plant Biotechnology

"Metabolomic profile of soil watered with residual water"

oscar.fernandez@cinvestav.mx